yyaE [2019-09-03 09:45:19]

similar to formate dehydrogenase

locus: BSU_40930

pI: 5.16

mw: 73.75 kDa

protein length: 667 aa

gene length: 2004 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]

Gene

Coordinates: 4,201,070 → 4,203,073

The protein

Protein family

• [SW|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)

Paralogous protein(s)

• [protein|628CCADB3C9A5F8A128C44ADE1DFDC2FC6B3CD01|YoaE]

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|2VPW] (from Thermus thermophilus, 26% identity) [pubmed|18536726]

Expression and Regulation

Operons

• genes: [gene|BD162A17E5F3F9AB49E478CE72D0FE92D82E6673|yyzM]-[gene|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|yyaE]-[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B870 (yyaE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1869 NBRP B. subtilis, Japan]
• BKE40930 (Δ[gene|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|yyaE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGCCGTCTTCTCCTTAC, downstream forward: _UP4_TAATCAAAGGGATTGGCAAG
• BKK40930 (Δ[gene|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|yyaE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGCCGTCTTCTCCTTAC, downstream forward: _UP4_TAATCAAAGGGATTGGCAAG

References

Research papers

• 18536726